Production of keratinase enzyme by Thermophilic Streptomyces Thermovulgari [electronic resource].

Includes references.

Seventeen thermophilic actinomycete isolates were tested for their keratinolytic activities. Results indicate that five isolates have keratinase activity. Streptomyces thermo vulgaris proved to be superior in keratinase formation and it was selected for the subsequent investigation. The optimum conditions for keratinase production and feather solubilization of S. thermovu/garis were studied. Data revealed that S thermovulgaris grew better with good yield of keratinase activity by growing it on the fermentation medium (100m/500ml flasks), in which the nitrogen and the carbon sources were replaced with sterile chicken feather pieces (1.5g%), 1.0g% starch and 0.lg% KzHPO4, using tap water with a homogenized spore suspension (containing approximately 8.2x 10-3 spores mtl ) of 3 days old culture. Inoculated flasks were incubated at 50°C for 4 days under static conditions.

Summary in Arabic.

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