Solubilization, purification, and characterization of the ATPase from spinach leaves plasma membrane fraction and its sensitivity to herbicides (Record no. 48647)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 02099cab a2200301Ia 45 0 |
| 001 - CONTROL NUMBER | |
| control field | u195506 |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | SIRSI |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 111011s2005 ua ss b eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EAL |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | eng |
| Language code of summary or abstract | ara |
| 090 ## - LOCALLY ASSIGNED LC-TYPE CALL NUMBER (OCLC); LOCAL CALL NUMBER (RLIN) | |
| Classification number (OCLC) (R) ; Classification number, CALL (RLIN) (NR) | ART AAR V10 No1 16 |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | El-Kassabany, Shafika A. |
| 240 10 - UNIFORM TITLE | |
| Uniform title | Journal of the advances in agricultural researches, 2005 v. 10 (1) |
| Medium | [electronic resource]. |
| 245 10 - TITLE STATEMENT | |
| Title | Solubilization, purification, and characterization of the ATPase from spinach leaves plasma membrane fraction and its sensitivity to herbicides |
| Medium | [electronic resource]. |
| 246 15 - VARYING FORM OF TITLE | |
| Title proper/short title | فصل وتنقية إنزيم الأدينوزين ثالث الفوسفاتيز من الغشاء السيتوبلازمى لخلايا أوراق السبانخ دراسة خصائصة وتاثير مبيدات الحشائش عليه. |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | P. 259-268. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc. note | Includes references. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The plasma membrane ATPase of spinach leaves (Spinacia oleracea) bad been solubilized with a two-step procedure using the anionic detergent, deoxycbolate (DOC) and the non ionic detergent, aminoxid WS35 as follows: I) loosely bound membrane proteins are removed by treatment with 0.1% DOC; 2) The ATPase is solubilized with 0.1 % aminoxid WS35 in the presence of 1 % DOC; 3) the solubilized material is further purified by centrifugation through a glycerol gradient (45-70%). Typically, about 10% of the ATPase activity is recovered, and the specific activity increases about II-fold. The properties of the purified ATPase are essentially the same as those of membrane-bound ATPase, with respect to substrate specificity, inhibitor sensitivity, and ion stimulation. |
| 546 ## - LANGUAGE NOTE | |
| Language note | Summary in Arabic. |
| 596 ## - | |
| -- | 1 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Plant cell walls. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Solubilization. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Adenosine triphosphatase. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Herbicides |
| General subdivision | Application. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name entry element | Spinach |
| General subdivision | Composition. |
| 773 0# - HOST ITEM ENTRY | |
| Title | Journal of the Advances in Agricultural Researches. |
| Related parts | 2005.v.10(1) |
| International Standard Serial Number | 1110-5585 |
| Control subfield | nnas |
| Record control number | u158404 |
| 856 40 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="http://nile.enal.sci.eg/EALE/2005/AAR/1005/1/259.pdf">http://nile.enal.sci.eg/EALE/2005/AAR/1005/1/259.pdf</a> |
| Public note | Full Text Article |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Articles |
| Source of classification or shelving scheme | Library of Congress Classification |
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