High alkaloids promising induced mutants by gamma rays and their molecular markers in Atropa belladonna L. [electronic resources].

Includes reference.

This investigation was carried out to induce gamma rays mutants in Atropa belladonna L. possessing high alkaloids contents. The used gamma rays doses were 50, 80, 110 and 150 Gy. The mutants had apparent morphological changes in plant height, no. of leaves and flowers as well as larg leaf area. Three promising high alkaloids mutants were selected from M2' M-11-1, M-11-2 and M-15-1. These promising mutants seemed to be a very important for their high alkaloids content, they possessed twice values than the control .These high alkaloids mutants, possessed stable morphological criteria at M3 generation. Molecular studies on these mutants were done for identification of them by ISSR technique confirming the difference between these mutants and control. The three mutants were distiguished by unique molecular markers, i.e. Mutant M-11-1 distinguished by three molecular markers with molecular sizes 1397, 1149 and 874 bp (base pair). Mutant M-11-2 distinguished by four molecular markers with molecular sizes 1537, 1075, 839 and 510 bp. Mutant M-15-1 distinguished by three molecular markers with molecular sizes 1749, 817and 756 bp. These findings drew the attention to the importance of genetic variation between these mutants and mother genotype, as well as, it considered a primary study to finger printing them.

Summary in Arabic.

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