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Evaluation of breeding programs susceptibility for two important forage crops using DNA barcoding [electronic resource]

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Bondok, Abdel Aziz. T

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Language: English Summary language: Arabic Description: P. 28-37Other title:

تقييم قابلية نوعين من محاصيل العلف لبرامج التربية باستخدام تقنية DNA Barcoding [Added title page title]

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Alexandria science exchange journal, 2017 v. 38 (1) [electronic resource]:

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Full Text Article

In: Alexandria Science Exchange Journal 2017.v.38(1)Summary: This investigation was carried out to identify and evaluate Medicago sativa and Trifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively. Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.

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Previous	ART ASEJ V38 No1 16 Investigation of copper removal from aqueous solution by cement kiln dust as industrial by-product	ART ASEJ V38 No1 2 The effect of blends between some Egyptian cotton varieties and introduced cottons on technological and spinning characters	ART ASEJ V38 No1 3 Morphological and biochemical markers for genetic diversity and salt tolerant In some barley cultivars and lines	ART ASEJ V38 No1 4 Evaluation of breeding programs susceptibility for two important forage crops using DNA barcoding	ART ASEJ V38 No1 5 Dissipation behavior of thiophanate-methyl in apple and strawberry fruits under open field conditions	ART ASEJ V38 No1 6 Molecular characterization of some Egyptian cotton varieties	ART ASEJ V38 No1 7 Response of some wheat cultivars to nano- , mineral fertilizers and amino acids foliar application	Next

Includes references.

This investigation was carried out to identify and evaluate Medicago sativa and Trifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively. Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.

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