TY  - SER
AU  - Noseir,W.M.B.
TI  - Effect of culture system on the survival of cryopreserved bovine embryos
KW  - Fertilization in vitro
KW  - Reproductive technology
KW  - Cows
KW  - Reproduction
KW  - Cell culture
KW  - Technique
KW  - Cattle
KW  - Genetics
N1  - Includes reference
N2  - This study compares the effect of culture system on the survival and hatching rates of in-vitro produced bovine embryos cryopreserved by slow freezing and vitrification. Oocytes were collected by slicing of ovaries from slaughtered cows. Presumptive zygotes produced by IVMIIVF were randomly allocated to one of the following culture media; TCM-l99 or SOP. Day 7 and day 8 blastocysts, cultured in TCM-l99 or SOF, were subjected to two cryopreservation methods; slow freezing and vitrification. Development of blastocysts 4, 24 and 48 hours post-thawing and hatching rates were recorded. There were no statistical differences in the post-thawing development or hatching rate between day 7 and day 8 cryopreserved blastocysts cultured in TCM-l99 or SOF
UR  - http://nile.enal.sci.eg/EALE/2008/VMJ/5608/1/61.pdf
ER  -