Application of lmmunohistochemistry to detect camelpox virus in cell culture and histological sections [electronic resource].
استخدام المناعة الكيميائية النسيجية للكشف عن فيروس جدري الجمال في المزارع الخلوية والشرائح النسيجية.
- p.209-222
Includes reference.
During the spring and summer of 2005, an outbreak of camelpox was spread in the province of Hama and Duma city. Therefore, this study was designed to detect camelpox virus in Vero cell culture and paraffm tissue section by immunohistochemistry. Skin samples (pustules, vesicles and scabs) from infected camels were collected from Duma and Hama. Monoclonal antibody (mouse anti-camelpox IgG) and labeled secondary antibody (rabbit anti-mouse IgG HRP) were used to demonstrate cainelpox virus. Camelpox virus was localized in the cytopathic effects in cell culture (syncytia and round cells) and inclusion bodies in the dermal and epidermal cell of the skin. The present study shows that immunohistochemistry is a sensitive diagnostic technique todetect the infection in skin samples with sensitivity of 98% and reflects its effectiveness and accuracy in camelpox infection diagnosis.