TY  - SER
AU  - Soliman,Y.A.
TI  - Molecular cloning and expression of ESAT-6 antigen of Mycobacterium bovis for differential diagnosis of bovine tuberculosis
KW  - Tuberculosis in cattle
KW  - Mycobacterium bovis
KW  - Molecular cloning
KW  - Tuberculosis
KW  - Microbiology
KW  - Enzyme-linked immunosorbent assay
KW  - Diagnosis
KW  - Antigen-antibody reactions
N1  - Includes references
N2  - In a search for developing new skin test reagents, the possibility of using ESAT-6 protein antigen as a candidate antigen for the diagnosis of bovine tuberculosis was investigated. Genomic DNA of M. bovis was extracted, purified and the esat-6 gene was amplified by PCR. The gene was then ligated to an expression vector, PQE. After transformation of an E. coli TOPO host strain with the PQE plasmid, the expression was induced using 10 mM of IPTG. A band (18 KDa) was seen in the SDS-PAGE analysis. The His-tagged r ESAT-6 antigen was then purified by metal affinity chromatography using Ni-NTA agarose
UR  - http://nile.enal.sci.eg/EALE/2007/ZVJ/3507/1/122.pdf
ER  -