Abd-Allah, S. M. <br/><br/>
Successful production of buffalo embryos from vitrified immature  and mature oocytes in vitro  [electronic resource]. 
 إنتاج ناجح لاجنة الجاموس من بويضات غير ناضجة وناضجة مزججة بالتجميد السريع معمليا. 

 - p.332-343. 
<br/><br/>Includes references. 
<br/><br/> The current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes romplexes (COCs) wm obtained from ovaries whil>h collel>ted from l! local abattoir Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours ofCOCs culture oocytes were vitrified similarly. All oocytes (immature and marure) were exposed to vitrification solution of different concentnltions (glycerol: 3.3,6.6 and 1000Ao) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and Immediately loaded in 0.25 ml plastic stnlws, each containing 100 )ul of 10% sucrose in Modified phosphate buffer saline (M-PBS). 
<br/><br/><br/>Summary in Arabic. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Buffaloes -- Embryos<br/>Buffaloes--Reproduction.<br/>Oocytes.<br/>Cryopreservation--methods.