TY  - SER
AU  - Abd-Allah,S.M.
AU  - Gomaa,A.
AU  - Abd El-Gawad,E.M.M.
TI  - Successful production of buffalo embryos from vitrified immature  and mature oocytes in vitro
KW  - Buffaloes
KW  -  Embryos
KW  - Reproduction
KW  - Oocytes
KW  - Cryopreservation
KW  - methods
N1  - Includes references
N2  - The current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes romplexes (COCs) wm obtained from ovaries whil>h collel>ted from l! local abattoir Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours ofCOCs culture oocytes were vitrified similarly. All oocytes (immature and marure) were exposed to vitrification solution of different concentnltions (glycerol: 3.3,6.6 and 1000Ao) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and Immediately loaded in 0.25 ml plastic stnlws, each containing 100 )ul of 10% sucrose in Modified phosphate buffer saline (M-PBS)
UR  - http://nile.enal.sci.eg/EALE/2009/AVMJ/5509/122/332.pdf
ER  -