Darwish,Samah.F. <br/><br/>
Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma  [electronic resource]. 
 تقيم اختبار تفاعل البلمرة المتسلسل التقليدى  مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى. 

 - p.285-299. 
<br/><br/>Includes references. 
<br/><br/> The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays. 
<br/><br/><br/>Summary in Arabic. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Cows--Diseases.<br/>Semen--Analysis.<br/>Cattle--Health.