TY  - SER
AU  - Darwish,SamahF.
AU  - Amin,A.S.
TI  - Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma
KW  - Cows
KW  - Diseases
KW  - Semen
KW  - Analysis
KW  - Cattle
KW  - Health
N1  - Includes references
N2  - The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays
UR  - http://nile.enal.sci.eg/EALE/2011/AVMJ/5711/128/285.pdf
ER  -