TY  - SER
AU  - El-Kassabany,Shafika A.
TI  - Solubilization, purification, and characterization of the ATPase from spinach leaves plasma membrane fraction and its sensitivity to herbicides
KW  - Plant cell walls
KW  - Solubilization
KW  - Adenosine triphosphatase
KW  - Herbicides
KW  - Application
KW  - Spinach
KW  - Composition
N1  - Includes references
N2  - The plasma membrane ATPase of spinach leaves (Spinacia oleracea) bad been solubilized with a two-step procedure using the anionic detergent, deoxycbolate (DOC) and the non ionic detergent, aminoxid WS35 as follows: I) loosely bound membrane proteins are removed by treatment with 0.1% DOC; 2) The ATPase is solubilized with 0.1 % aminoxid WS35 in the presence of 1 % DOC; 3) the solubilized material is further purified by centrifugation through a glycerol gradient (45-70%). Typically, about 10% of the ATPase activity is recovered, and the specific activity increases about II-fold. The properties of the purified ATPase are essentially the same as those of membrane-bound ATPase, with respect to substrate specificity, inhibitor sensitivity, and ion stimulation
UR  - http://nile.enal.sci.eg/EALE/2005/AAR/1005/1/259.pdf
ER  -