TY  - SER
AU  - Fahmy,Eman M.
AU  - Abd El-Gawad,Nermin M.
AU  - El-Geddawy,I.H.
AU  - Saleh,O.M.
AU  - El-Azab,Nasra M.
TI  - Development of RAPD and ISSR markers for drought tolerance in sugarcane (Saccharum officinarum L.)
KW  - Sugarcane
KW  - Breeding
KW  - Genetics
KW  - Varieties
KW  - Drought tolerance
N1  - Includes references
N2  - Nine varieties of sugarcane (Saccharum officinarum L.) were screened for their water stress tolerance in field experiment. Analysis of variance showed significant differences for five yieldrelated traits among the nine varieties under control and drought treatments. The results indicated that varieties G.T.54-9, Co.997 and F153 were the most tolerant while varieties F161, NCo.310 and Phil.8013 were the most sensitive ones. RAPD- and ISSR-PCR techniques were used in this study to detect some molecular markers associated with drought tolerance in sugarcane. RAPDPCR results using eight random primers exhibited 59 amplified fragments; 48 of them were polymorphic (81.4%).The results indicated the presences of eight positive and two negative molecular markers for drought tolerance trait. ISSRPCR results with five primers showed 27 amplified fragments; 24 of them were polymorphic (88.8%). ISSR analysis exhibited five positive and two negative molecular markers for drought tolerance trait. Each technique was used to detect the similarity indices and the dendrogram of varieties relationships. However, the overall similarity indices based on both techniques revealed that the highest similarity was 83% between varieties; G.74-96 and Nco.310 followed by varieties; F.153 and F.161 (82%), while the lowest similarity was 53% between varieties; G.T.54-9 and POJ.28-78, followed by varieties; G.T.54-9 and F.153 (56%). The dendrogram resulting from the combination of the two systems separated the nine sugarcane varieties into two main clusters, where variety F.153 was placed in a separate cluster, while the second cluster involved the rest of the varieties. The second cluster was subdivided further into two sub-clusters. The first sub-cluster included variety Co.997 only, while the rest of the varieties occurred in a separate sub-cluster. The aforementioned results emphasized the advantages of using a combination of several molecular systems over the use of one system in order to obtain higher resolution to discriminate between different genotypes
UR  - http://nile.enal.sci.eg/EALE/2008/EJGC/3708/1/1.pdf
ER  -