TY  - SER
AU  - El-Bouhy,Z.
AU  - El-Nobi,G.
AU  - Hakim,Y.
TI  - Methods for genetic differentiation of Flavobacterium columnare isolated from some cultured freshwater fishes
KW  - Freshwater fishes
KW  - Diseases
KW  - Microbioiolgy
KW  - animal pathogenic bacteria
KW  - Flavobacterium columnare
KW  - genetic variation
N1  - Includes references
N2  - Flavobacterium columnare is an important economical pathogen that affects freshwater fishes. This study aimed to investigate the genetic variations among the isolated F columnare from naturally infected Clarias gariepinus, Oreochromis niloticus and Cyprinus carpio using randomly amplified polymorphic DNA analysis (RAPD) PCR and Polyacrylamide gel electrophoresis (PAGE) in the presence of the sodium dodecylsulfate (SDS). The affected fishes with F. columnare suffered from hemorrhagic ulcer in the skin, severe fin rot especially at the caudal fin and respiratory manifestations. On ordal's selective media, the isolates showed characteristic yellowish rhizoids colonies. The isolated F. columnare from different fish species had no clear significant genetic variations. However, the total amount of the protein which is present in the cell wall of each isolate was completely different
UR  - http://nile.enal.sci.eg/EALE/2011/ZVJ/3911/1/103.pdf
ER  -