Interactions of diluents, cryoprotective agents and straw filling capacity on quality and fertilizing ability of buffalo semen [electronic resource].
- p.553-566.
Includes references.
The present study was carried out on three buffalo bulls. Their semen was extended in three diluents (Milk, Laiciphos and Tris) with three cryoprotective agents (Glycerol, DMSO and propylene glycoL PG) packaged in either mini (0.25ml) or medi (0.50 ml) straws. Significant differences in the percentages of post-thawing motility (PTM), intact acrosome (PIA) and hypoosmotic swelling test (HOST) were observed for semen extended in Milk diluent (52.80±O.39%; 95.40±0.43% and 17.97±0.27%, respectively), Laciphos (48.33± 0.48%: 94.50 ±0.29% and 15.77±O.22%, respectively) and Tris (46.23 ± 0.49%; 92:13±0.37% and 14.43 ( 0.21% respectively). The semen quality test (SQT) appeared significantly higher for milk diluent (2.13±0.11) than Tris and Laiciphos diluents (1.77±0.05 and 1.72± 0.06, respectively).