01478cab a2200205Ia 4500001000800000003000600008008004100014040000800055100001500063240008400078245013500162300001200297504002500309520071300334650002201047653002401069700001601093773008701109856007601196u206868SIRSI110417s2001    ua     ss b         eng d  aEAL1 aTemsah, S.10aAlexandria journal of veterinary science, 2001 v. 17 (1)h[electronic reource].10aProduction, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidaseh[electronic reource].  ap.1-16.  aIncludes references.  aPenicillin amidase enzyme (PA) was isolated from the culture filtrate of one isolated strain of E. coli E-U₁₀ grown on optimized medium after 24 hours incubation. The enzyme was purified to homogeneity by sequential ammonium sulfate fractionation and ion exchange and gel filtration chromatography. On using Disc-PAGE, the final purification fraction showed only one distinctive band indicating high purity. On using SDS-PAGE the final fraction showed two distinctive units with molecular weight of 22000 and 59000 Dalton. The optimal pH, temperature and time course for activity were 7.5, 37°C and 10 minutes respectively. The enzyme with penicillin G as substrate had a Km of 13 mM and Vmax 3.92 U/ml. 0aEscherichia coli.  apenicillin amidase.1 aMabrouk, A.0 tAlexandria Journal of Veterinary Science. g2001.v.17(1)x1110-20477nnaswu19134340uhttp://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdfzFull Text Article.