01555cab a2200241Ia 45 0 u206868 SIRSI 110417s2001 ua ss b eng d EAL ART AJVS V17 No1 1 Temsah, S. Alexandria journal of veterinary science, 2001 v. 17 (1) [electronic reource]. Production, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidase [electronic reource]. p.1-16. Includes references. Penicillin amidase enzyme (PA) was isolated from the culture filtrate of one isolated strain of E. coli E-U₁₀ grown on optimized medium after 24 hours incubation. The enzyme was purified to homogeneity by sequential ammonium sulfate fractionation and ion exchange and gel filtration chromatography. On using Disc-PAGE, the final purification fraction showed only one distinctive band indicating high purity. On using SDS-PAGE the final fraction showed two distinctive units with molecular weight of 22000 and 59000 Dalton. The optimal pH, temperature and time course for activity were 7.5, 37°C and 10 minutes respectively. The enzyme with penicillin G as substrate had a Km of 13 mM and Vmax 3.92 U/ml. Escherichia coli. penicillin amidase. Mabrouk, A. Alexandria Journal of Veterinary Science. 2001.v.17(1) 1110-2047 nnas u191343 http://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdf Full Text Article. 1 AR lcc 57052 57052