TY  - SER
AU  - Temsah,S.
AU  - Mabrouk,A.
TI  - Production, purification, characterization and immunological studies of E.coli E-U₁₀ penicillin amidase
KW  - Escherichia coli
KW  - penicillin amidase
N1  - Includes references
N2  - Penicillin amidase enzyme (PA) was isolated from the culture filtrate of one isolated strain of E. coli E-U₁₀ grown on optimized medium after 24 hours incubation. The enzyme was purified to homogeneity by sequential ammonium sulfate fractionation and ion exchange and gel filtration chromatography. On using Disc-PAGE, the final purification fraction showed only one distinctive band indicating high purity. On using SDS-PAGE the final fraction showed two distinctive units with molecular weight of 22000 and 59000 Dalton. The optimal pH, temperature and time course for activity were 7.5, 37°C and 10 minutes respectively. The enzyme with penicillin G as substrate had a Km of 13 mM and Vmax 3.92 U/ml
UR  - http://nile.enal.sci.eg/EALE/2001/AJVS/1701/1/1.pdf
ER  -