01945cab a2200301Ia 45 0 57723 57723 u207778 SIRSI 141210s2013 ua ss b eng d EAL engbara ART AVMJ V59 No138 14 Zakary, Eman M. 1029 Assiut veterinary medical journal, 2013 v. 59 (138) [electronic resource]. Comparison between traditional methods and real time PCR for detection of E .coli in bovine meat products [electronic resource]. مقارنة بين الطرق التقليدية وتفاعل انزيم البلمرة المتسلسل ذو الوقت الحقيقى فى كشف الاشريشيا كولاى فى منتجات اللحوم البقرية. p.103-114. Includes references. This study was performed to compare the prevalence of E .coli in minced meat and sausage using traditional methods and real time PCR. A total of 100 samples of minced meat and sausage (50 of each) were collected from different supermarket in Giza and Assiute governorates. The results using traditional methods recorded as 25% and 15% as positive E .coli from minced meat and sausage respectively, while the incidence of E. coli 0157 recorded as 4% and 2% from minced meat and sausage respectively. The incidence of E. coliO 157 by using real time PCR recorded as 8% and 6% from minced meat and sausage respectively. The melting curve appeared at (82 o C) for E. coli 0 157 by using SYBR green Real time PCR. Summary in Arabic. Meat products. 1045 Meat Microbiology 1046 Polymerase chain reaction. 250 Escherichia coli O157:H7 120 Fouad, Marium 747 Assiut Veterinary Medical Journal. 2013.v.59(138) 1012-5973 nnas u181356 http://nile.enal.sci.eg/EALE/2013/AVMJ/5913/138/103.pdf Full Text Article. AR lcc