Significance of herbaspirillum seropedicae inoculation and (or) maize straw amendment on nitrogen fixation and growth of wheat using ¹⁵N -dilution technique [electronic resource].
دراسة اهمية التلقيح ببكتريا الهرباسبيرلام سيرووبدكا في وجود أو غياب قش الذرة علي تثبيت النيتروجين ونمو القمح باستخدام تقنية تخفيف النيتروجين.
- p.501-513.
Includes references.
Pot experiments were conducted to investigate the significance of Herbaspirilltum seropedicae (278) inoculation and (or) maize straw (0, 5 and 10 T ha-l ) amendment on growth and nitrogen fixation of wheat using ¹⁵N-dilution technique. Inoculation resulted in accumulation of fixed nitrogen and %nitrogen derived from atmosphere (NdFa) recorded 24.6% and 26.5% in wheat shoots and grains, respectively. Straw amendment reduced %NdFa to 16.1% and 20.2% at high straw level (10 T ha-I). Rational nitrogen fertilization at rate of 180 Kg N ha·l completely inhibited nitrogen fixation by Herbaspiril/um inoculation. Bacterial inoculation increased dry shoots and grains yields up to 23% and 31% respectively. The highest levels of shoots and grains dry mass (46.5% and 42.4%, respectively) were obtained by Nfertilization in both inoculated and uninoculated plants. Total shoots and grains Nyields were increased irrespective of organic matter amendment by H. seropedicae inoculation up to 9% and 25% respectively. N-fertilized plants recorded a maximum increase in N-yield (57% and 51%). H. seropedicae was reisolated from inoculated wheat histosphere after harvesting (90 days from sowing). Neither organic matter nor mineral nitrogen applications had marked effect on bacterial total counts colonized wheat histosphere. Moreover, no symptoms of mottled stripe disease were observed on leaves and stems of inoculated plants.