02165cab a2200337Ia 45 0 u208761 SIRSI 150217s2002 ua ss b eng d EAL eng ara ART AJB V5 No1 1 Ibrahim, M.A. Arab journal of biotechnology, 2002 v. 5 (1) [electronic resource]: an international journal devoted to the advancement of biotechnology. Genetically modified potato (Solanum tuberosum L.) resistant to potato tuber moth (Phthorimaea operculella) [electronic resource نباتات بطاطس معدلة وراثيا لمقاومة فراشة درنات البطاطس p.1-10 Includes references. Potato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively. Summary in Arabic. Potatoes Genetics Potatoes Breeding Potatoes Diseases and pest resistance Potato tuberworm Biological control Metry, E.A. Osman, Y.A. Nasr El-Din, T.M. Madkour, M.A. Arab Journal of Biotechnology. 2002.v.5(1) 1110-6875 nnas u189256 http://nile.enal.sci.eg/EALE/2002/AJB/502/1/1.pdf Full Text Article 1 AR lcc 58470 58470