Ibrahim, M.A. creator Metry, E.A. Osman, Y.A. Nasr El-Din, T.M. Madkour, M.A. text bibliography ua 2002 continuing eng ara

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p.1-10 Potato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively. Includes references. Summary in Arabic. Potatoes Genetics Potatoes Breeding Potatoes Diseases and pest resistance Potato tuberworm Biological control 1110-6875 u189256 2002.v.5(1) http://nile.enal.sci.eg/EALE/2002/AJB/502/1/1.pdf http://nile.enal.sci.eg/EALE/2002/AJB/502/1/1.pdf EAL 150217 u208761