02194cab a2200349Ia 4500001000800000003000600008008004100014040000800055041001300063090002100076100001900097240014500116245013400261246010400395300001100499504002500510520090100535546002301436650002301459650002301482650004301505650004101548700001601589700001601605700002201621700001801643773007501661856007301736596000601809942001201815999001701827u208761SIRSI150217s2002    ua     ss b         eng d  aEAL  aengbara  aART AJB V5 No1 11 aIbrahim, M.A. 10aArab journal of biotechnology, 2002 v. 5 (1)h[electronic resource]:b an international journal devoted to the advancement of biotechnology.10aGenetically modified potato (Solanum tuberosum L.) resistant to potato tuber moth (Phthorimaea operculella)h[electronic resource15aنباتات بطاطس معدلة وراثيا لمقاومة فراشة درنات البطاطس  ap.1-10  aIncludes references.  aPotato tuber moth (PTM), Phthorimaea operculella, causes tremendous economic losses to potato production in Egypt. An Egyptian isolate of the entomopathogenic bacteria Bacillus thuringiensis (Bt), isolate CI2, produces a potent Cry1Aa7 toxin that kills the larval stages of PTM, more efficiently than other standard Bt toxins. The cry1Aa7 gene cloned in the binary vector pBI121 and pBI-cry7 construct was transformed to Agrobacterium tumefaciens strain LBA4404 viaBtriparental mating. Transformed LBA4404 strains harboring pB1-cry7 were used to mobilize the cry1Aa7 cassette into the potato cultivar Desiree. Out of 91 transformants, five individual lines (D8, D9, D13, D26 and D37) were shown to be transgenic for cry1Aa7. The integrated toxin gene as well as the expressed Cry1Aa7 was confirmed using polymerase chain reaction (PCR) and enzyme-linked immunosorbant assay (ELISA), respectively.  aSummary in Arabic. 0aPotatoesxGenetics 0aPotatoesxBreeding 0aPotatoesxDiseases and pest resistance 0aPotato tuberwormxBiological control1 aMetry, E.A.1 aOsman, Y.A.1 aNasr El-Din, T.M.1 aMadkour, M.A.0 tArab Journal of Biotechnology. g2002.v.5(1)x1110-68757nnaswu18925640uhttp://nile.enal.sci.eg/EALE/2002/AJB/502/1/1.pdfzFull Text Article  a1  cAR2lcc  c58470d58470