02258cab a2200301Ia 4500001000800000003000600008008004100014040000800055041001300063100002200076240007200098245011000170246019400280300001500474504002500489520105000514546002301564650002201587650003101609650004701640650002201687700002601709700002201735700002101757700002501778773007601803856007701879u210715SIRSI110120s2012    ua     ss b         eng d  aEAL  aengbara1 aEl-Leithy, M. A. 10aEgyptian journal of microbiology, 2012 v.47h[electronic resource].10aDetection of six E.coli O157 virulence genes in water samples using multiplex PCRh[electronic resource].15aالكشف عن ستة جينات شديدة الاصابة فى الايشريشيا كولاى O157 فى عينات المياه باستخدام تفاعل البلمره المتعدد.  ap.171-188.  aIncludes references.  aEscherichia coli O157 strains have emerged as important human enteric pathogens. E.coli O157 strains may be transmitted in a variety of ways, including drinking water, recreational water and wastewater. One hundred and seventy five water samples were collected from different water sources from June 2010 to July 2011 and examined for classical bacterial indicators (total bacterial counts at 37°C and 22°C, total coliforms, fecal coliforms and fecal streptococci) and E.coli O157. Total coliforms (TC), fecal coliforms (FC) and fecal streptococci (FS) among the collected water samples were 83, 76 and 76 out of 175 (MPN- index/100 ml) with incidence ratio of 47%, 43% and 43%, respectively. Escherichia coli O157 was detected in water samples using HiCrome EC O157:H7 agar and multiplex PCR targeting six virulence genes (stxl (Shiga toxin 1 gene), stx2 (Shiga toxin 2 gene), eae (intimin gene), hlyA (hemolysin gene), rfbE (O157 antigen gene), and fliC (flagellar antigen gene)}.  Keywords: E.coli O157, Virulence gene, Multiplex PCR, Water  aSummary in Arabic. 0aEscherichia coli. 0aPolymerase chain reaction. 0aVirulence (Microbiology)xGenetic aspects. 0aWaterxPollution.1 aEl-Shatoury, Einas H.1 aEl-Senousy, W. M.1 aAbou-Zeid, M. A.1 aEl-Taweel, Gamila E.0 tEgyptian Journal of Microbiology. g2012.v.47x0022-27047nnaswu18401240uhttp://nile.enal.sci.eg/EALE/2012/EJM/4712/0/171.pdfzFull Text Article.