02331cab a2200337Ia 45 0 u210715 SIRSI 110120s2012 ua ss b eng d EAL eng ara ART EJM V47 11 El-Leithy, M. A. Egyptian journal of microbiology, 2012 v.47 [electronic resource]. Detection of six E.coli O157 virulence genes in water samples using multiplex PCR [electronic resource]. الكشف عن ستة جينات شديدة الاصابة فى الايشريشيا كولاى O157 فى عينات المياه باستخدام تفاعل البلمره المتعدد. p.171-188. Includes references. Escherichia coli O157 strains have emerged as important human enteric pathogens. E.coli O157 strains may be transmitted in a variety of ways, including drinking water, recreational water and wastewater. One hundred and seventy five water samples were collected from different water sources from June 2010 to July 2011 and examined for classical bacterial indicators (total bacterial counts at 37°C and 22°C, total coliforms, fecal coliforms and fecal streptococci) and E.coli O157. Total coliforms (TC), fecal coliforms (FC) and fecal streptococci (FS) among the collected water samples were 83, 76 and 76 out of 175 (MPN- index/100 ml) with incidence ratio of 47%, 43% and 43%, respectively. Escherichia coli O157 was detected in water samples using HiCrome EC O157:H7 agar and multiplex PCR targeting six virulence genes (stxl (Shiga toxin 1 gene), stx2 (Shiga toxin 2 gene), eae (intimin gene), hlyA (hemolysin gene), rfbE (O157 antigen gene), and fliC (flagellar antigen gene)}. Keywords: E.coli O157, Virulence gene, Multiplex PCR, Water Summary in Arabic. Escherichia coli. Polymerase chain reaction. Virulence (Microbiology) Genetic aspects. Water Pollution. El-Shatoury, Einas H. El-Senousy, W. M. Abou-Zeid, M. A. El-Taweel, Gamila E. Egyptian Journal of Microbiology. 2012.v.47 0022-2704 nnas u184012 http://nile.enal.sci.eg/EALE/2012/EJM/4712/0/171.pdf Full Text Article. 1 AR lcc 59917 59917