TY  - SER
AU  - Shaltout,A.D.
AU  - El-Wakeel,H.F.
AU  - Nahla,A.A.
AU  - Ghada,M.S.
TI  - Production of some new peach rootstock through hybridization and tissue culture technique
KW  - Peach
KW  - Production
KW  - Hybridization
KW  - Mutation breeding
KW  - Plant tissue culture
KW  - Nematode diseases of plants
KW  - Root-knot nematodes
KW  - Control
N1  - Includes references
N2  - This study was carried out through three succeeded years (2005- 2007)  in  almond-  peach  orchard  aimed  to  grow  plantlets  of  new   hybrids  coming  from  hybridization   between  both  of  Om  El-fahm  X   Hansen  by  using  tissue  culture  technique  and  to  determine  some   molecular  markers  related  to  root -knot  nematode  resistance  were   detected. In field experiment, height  of hybrids seedlings ranged from  53.3-70.1 cm, 2.21- 2.65 cm for stem thickness and 41 -63 for leaves  number  per  one.  As  for  tissue  cu lture  experiment,  stem  node  was   superior  than  shoot  tip  on  bud  development  and  shoot  length  in   establishment  stage.  Meanwhile,  th e  third  subculture  records  the   greatest  significant  number  of  produced  shoots  and  shoot  length  in   multiplication  stage.  for  rooting  stage,  all  genotype  hybrids  raised   mediated ranged from 53.3-73.3 &4.16-5.33 and 3.14-5.11 for rooting  %, root number/ shoot and root le ngth, respectively.  Hybrid genotype  data  confirmed  that  hybrid  no.4  wa s  superior  than  the  other  hybrids.   By  using  four  primers  pairs  relate d  to  root  knot  nematode  resistance   by two techniques. Pchgms primer by SSR and OPA11, OP834B and  OPAP4  by  STS  were  used.  Four   molecular  markers  appeared  in   hybrid  no.  4  only  which  may  be  the  mo st  hybrid  can  be  resistant  to   This study was carried out through three succeeded years (2005- 2007)  in  almond-  peach  orchard  aimed  to  grow  plantlets  of  new   hybrids  coming  from  hybridization   between  both  of  Om  El-fahm  X   Hansen  by  using  tissue  culture  technique  and  to  determine  some   molecular  markers  related  to  root -knot  nematode  resistance  were   detected. In field experiment, height  of hybrids seedlings ranged from  53.3-70.1 cm, 2.21- 2.65 cm for stem thickness and 41 -63 for leaves  number  per  one.  As  for  tissue  cu lture  experiment,  stem  node  was   superior  than  shoot  tip  on  bud  development  and  shoot  length  in   establishment  stage.  Meanwhile,  th e  third  subculture  records  the   greatest  significant  number  of  produced  shoots  and  shoot  length  in   multiplication  stage.  for  rooting  stage,  all  genotype  hybrids  raised   mediated ranged from 53.3-73.3 &4.16-5.33 and 3.14-5.11 for rooting  %, root number/ shoot and root le ngth, respectively.  Hybrid genotype  data  confirmed  that  hybrid  no.4  wa s  superior  than  the  other  hybrids.   By  using  four  primers  pairs  related  to  root  knot  nematode  resistance
UR  - http://nile.enal.sci.eg/EALE/2012/JBCES/712/1/361.pdf
ER  -