TY  - SER
AU  - Abdel-Ghani,M.A.
AU  - Suzuki,H.
TI  - Effect of cumulus cells on canine oocyte vitrification using DAP213 cryotop method
KW  - Dogs
KW  - Reproduction
KW  - Germplasm resources
KW  - Cryopreservation
KW  - Oocytin
KW  - cumulus oophorus
N1  - Includes references
N2  - The present study investigated the influence of cumulus cells on vitrification of canine germinal vesicle stage oocytes, and assessed the ability of vitrified -warmed oocytes for further maturation in vitro. Selected cumulus-oocyte complexes (COCs) or cumulus-free oocytes or partially denuded were vitrified in DAP 213 solution. The vitrified-warmed oocytes were observed morphologically, and the plasma integrity was assessed. The viable oocytes in each group were cultured in TCM-199 for 72 h at 38.5 oc in 5% C02, 5% 02 and 90% N2. All media were supplemented with 10% ofFCS, 50 ng/mL of EGF, 2 Ug/mL of estradiol-17?, 0.1 IU/mL ofhCG, 0.1 IU/mL of FSH, 0.25 mM of pyruvic acid, 100 UM of ?-mercaptoethanol, and antibiotics. The viability of vitrified-warmed intact oocytes (52.8%) was higher than denuded oocytes (33.9%, P < 0.05), however, there is no differences between the partially denuded oocytes (43.9%) and denuded oocytes (33.9%, P > 0.05)
UR  - http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/142/46.pdf
ER  -