TY  - SER
AU  - El-Leboudy,Ahlam A.
AU  - Saad,Nagah M.
AU  - Amer,A.
AU  - Elmoazen,Noha S.
TI  - Multiplex PCR and latex agglutination for identification and characterization of toxigenic clostridium difficult from raw milk
KW  - Raw milk
KW  - Clostridium diseases
KW  - Milk
KW  - Microbiology
KW  - Polymerase chain reaction
KW  - Agglutination tests
KW  - Clostridium difficile
KW  - PCR
N1  - Includes references
N2  - Community-acquired C.Diff infections have recently been increasing in incidence and severity. Several studies have isolated C. Diff spores from livestock and food suggesting that food may play a role in transmission. The overall goals of this research were to: 1) determine prevalence of C. Diff from raw milk using selective (cycloserine-cefoxitin fructose agar [CCFA]; 2) determine toxigenic profiles of C.Diff. isolated using PCR targeting tpi and tcdB genes. A total of 50 random samples of raw marketable milk were collected from different localities in Assuit city, Egypt. The obtained results indicated that about 11 (22%) out of 50 examined raw milk samples were positive for C.Diff using conventional method. While, 2 strains of C.Diff. (18.2%) out of the positive 11 strains could be detected using the latex agglutination test.   Keywords: Clostridium Difficile, Raw milk, latex agglutination, toxigenic, PCR
UR  - http://nile.enal.sci.eg/EALE/2014/AVMJ/6014/143/49.pdf
ER  -