TY  - SER
AU  - Shaaban,M.N.
AU  - Osman,M.A.
AU  - Mohamady,Aziza H.
TI  - Effect of some insecticides on genomic dna of laboratory and resistant strains of Spodoptera Littoralis
KW  - Insecticides
KW  - Toxicology
KW  - Spodoptera littoralis
KW  - Larvae
KW  - Control
KW  - Genomic Instability
KW  - Genetics
KW  - Insects
KW  - Laboratory manuals
KW  - Random amplified polymorphic DNA technique
N1  - Includes references
N2  - The RAPD technique was used as a tool for setting up a convenient and standard protocol to determine the interspecific variation between the two strains (laboratory and resistant) of Spodoptera littoralis and estimate the intraspecific variation within each strain after treatment. Five (OPA-7, 10, 16, OPB-15 and 20) out of ten primers gave clearly differences among the two strains on the basis of the amplified product patterns, These primers generated 296 fragments The comparison between the two strains of S. littoralis showed differences in the sizes and numbers of the amplified fragments per primer for each strain and each treatment, indicating a high degree of variability between them. The primers OPA-7,16, OPB-15 and 20 gave band patterns which revealed that, there was some degree of divergence between the genome of untreated and treated larvae, higher than primer OPA-l0
UR  - http://nile.enal.sci.eg/EALE/2007/EJAR/8507/4/1313.pdf
ER  -