TY  - SER
AU  - Hussein,Ebtissam H.A.
AU  - Madkour,Magdy A.
AU  - Assem,Shireen K.
AU  - Radwan,Al-Zahraa M.A.
TI  - Embryogenic callus formation and plant regeneration from immature embryos of some barley genotypes (Hordeum vulgare L.)
KW  - Barley
KW  - Genetic engineering
KW  - Genetics
KW  - Tissue culture
KW  - Technique
KW  - Behavioral embryology
KW  - Somatic embryogenesis
KW  - Embryology
KW  - Varieties
N1  - Includes references
N2  - Genotypic restrictions on plant regeneration from cultured cells have hindered the genetic transformation of most barley cultivars. Optimizing culturing protocols for some genotypes may facilitate their genetic transformation to produce transgenic plants with desired characters. Plant regeneration from embryogenic callus of six barley genotypes (Hordeum vulgare L.) was examined on four different media compositions. Regeneration was improved for all genotypes by separately autoclaving certain components of the culture media and using maltose as the carbon source. Medium No.2 revealed the highest frequency of embryogenic calli (74.56%), shoots (26.58%) and regenerated plantlets (14.33%) across all genotypes. Genotypes Giza 124, Giza 126 and breeding line No. 6 revealed the highest regeneration frequency (9.83%, 9.53% and 10.70%, respectively).Using the intact or bisected immature embryos, as explants did not significantly affect the regeneration frequency of all genotypes
UR  - http://nile.enal.sci.eg/EALE/2004/AJB/704/1/111.pdf
ER  -