TY  - SER
AU  - Mohamed,A.H.
AU  - Carro,M.D.
AU  - Mohamed,S.A.
AU  - Ranilla,M.J.
TI  - Influence of treating some low-quality forages with exogenous fibrolytic enzymes on ruminal fermentation
KW  - Rumen fermentation
KW  - Forage
KW  - Cellulase
KW  - Xylanases
N1  - Includes references
N2  - A study was undertaken to assess appropriate fibrolytic enzymes inclusion effects of three fibrolytic enzymes treatments on the In vitro ruminal fermentation of five low-quality forages (three cereal straws (barley, wheat and rice), com stover and Rhodes grass hay) were investigated using batch cultures of mixed ruminal microorganisms. Four different treatments were investigated: no additive (control; CON), cellulase from Aspergillus niger (CEL; Flub Chemie GmbH), xylanase from ruminal microorganisms (XYL; Megazyme International Ireland Ltd), and a 1: 1 mixture cellulase:xylanase (MIX). Enzymes (20 IU/g forage dry matter (DM)) were applied directly into the forages 24 h before incubation with buffered ruminal fluid at 39C for 24 h. The treatment with CEL increased (P<0.05) gas production after 24 h of incubation for wheat straw, barley straw and grass hay. Meanwhile, the treatment with MIX and XYL did not affect (P>0.05) gas production for all forage. For all forages, there was no change (p>0.05) in NH3-N concentration with added enzymes, indicating no differences in protein degradability and/or ammonia-N incorporation by ruminal microorganisms. Also, Natural detergent fiber degradability (NDFD) concentration were not affected (P>0.05) by the addition of enzymes. The treatment of low-quality forages with CEL increased the production of propionate for Rhodes grass hay, com stover and barley straw, and increased (P<0.05) total VFA production for wheat straw and com stover. No effects (p>0.05) of MIX and XYL treatments on VFA production were observed for any substrate. Under the conditions of the present experiment, the pre-treatment of low-quality forages with cellulase and xylanase enzymes produced subtle effects on In vitro ruminal fermentation, suggesting that the used enzymes contributed little, if any, to ruminal fibrolytic activity
UR  - http://nile.enal.sci.eg/EALE/2009/EJNF/1209/2/207.pdf
ER  -