TY  - SER
AU  - Nawar,Mohamed A.
AU  - Abo-Elnasr,Amany A.
AU  - Kobisi,Abdel Nasser A.
AU  - Hefnawy,Gohyza A.
TI  - Evaluation of Acaricidal activity of Purpureocillium Lilacinum isolated from Egyptian soil against Tetranychus Urticae
KW  - Two-spotted spider mite
KW  - Biological Control
KW  - Paecilomyces lilacinus
N1  - Includes references
N2  - The study is aimed to isolate and identify a potential entomopathogenic fungus from fields infested with spider mites and evaluate 
them as a bio-rationale agent against Tetranychus urticae Koch., which is an important agricultural pest that infests horticultural 
crops in both field and greenhouses. Macroscopic and microscopic characteristics showed that the obtained entomogenous isolate was 
Purpureocillium lilacinum (formerly, Paecilomyces lilacinus). P. Lilacinum demonstrated mortality rates on adult females of T. urticae 
of 71.19 and 77.97% with conidial concentrations of 5x107 and 1.6 x108, respectively, 10 days after application. Median lethal concentration 
of P. lilacinum was 2.85 106 conidia/ml. Enzymatic activity of P. lilacinum was evaluated. In this context, chitinolytic activity 
of P. lilacinum was relatively weak, since the clear halo obtained was only 4.43 mm in diameter. The activity of chitinase enzyme 
insignificantly increased over the incubation period of 10 days. In contrast, proteolytic activity of P. lilacinum was high and 
showed a clear zone (25.8 mm) around the colony after 10 days. So, it is concluded that P. lilacinum has a potential biological 
control against T. urticae. Finally, evaluation of the ethyl acetate extract of P. lilacinum showed acaricidal potency since it 
exhibited LC50 values of 10.49 mg/ml for eggs and 30.75 mg/ml for adults. So, it is concluded that P. lilacinum has a potential 
biological control against T. urticae

UR  - http://nile.enal.sci.eg/EALE/2018/EJDR/6818/2/157.pdf
ER  -