nab a22 a 4500 0 0 alc 0 0 ENAL ENAL 2020-02-03 0 ART AJVS V53 No2 6 2020-02-03 00:00:00 2020-02-03 AR 65568 65568 ENAL 030220s2017 ua n ss b a eng d EAL EAL eng ara ART AJVS V53 No2 6 Abotalp, Eman H. 26810 Alexandria journal of veterinary science, 2017 v. 53 (2) [electronic reource]. Molecular detection of virulence genes of escherichia coli o157 isolated from different sources [electronic reource]. p. 38-44 Includes references. Escherichia coli O157 is recognized as an important foodborne pathogen responsible for sporadic cases to serious outbreaks worldwide. The morbidity and mortality associated with several recent outbreaks due to STEC have highlighted the threat this organism possess to public health. Bacteriological examination of 671 samples collected from cattle, sheep, chickens, ducks, milk and water showed that 34.3% (230/671) were positive E. coli. Out of 230 isolates of positive E. coli, (16) isolate were identified by vitek2 system as E. coli O157 (6.9%) with high incidence in cattle samples (20%) then chicken and water samples (13.6%) and (12.5%) respectively. On the other hand, there were no isolates from ducks samples for E. coli O157 and with low percent (7.8%) from raw milk samples and (1%) from sheep samples. The application of PCR for screening of virulence genes (sxt1, sxt2 and eaeA) in E. coli O157 isolates revealed that out of 16 isolates of E. coli O157 there were (9), (6) and (16) were positive for stx1, stx2 and eaeA, respectively. This study concluded that molecular identification by PCR for detection of E. coli O157 virulence genes is rapid, specific and accurate approach in studying the actual role of these genes in disease pathogenicity. Summary in Arabic Escherichia coli O157:H7 30534 Escherichia coli O157:H7 Analysis 30535 Abdeen, Eman 30536 Moustafa, Alaa El-Din H. 30537 Mohamed, Sahar R. 30538 Alexandria Journal of Veterinary Science. 2017.v.53(2) 1110-2047 nnas u191343 http://nile.enal.sci.eg/EALE/2017/AJVS/5317/2/38.pdf Full Text Article. AR alc