Atwa, Marwa Th. <br/><br/>
Effect of cryptosporidium parvum infection on DNA damage and cytokines gene expression in blood of immunocompromised mice  [electronic resource]: 
 تأثير العدوي بالطفيلي " Cryptosporidium parvum " علي السمية الوراثية والتعبير الجيني للسيتوكينات فى دم الفئران المثبطة مناعيا 

 - p. 16-25. 
<br/><br/>Includes bibliographic reference 
<br/><br/> There are few data regarding the genotoxic and immuno-modulatory effects of Cryptosporidium parvum (Family: Cryptosporidiidae) infection in vivo. <br/>Therefore, the present study evaluated the effect of Cryptosporidium parvum infection on DNA and gene expressions of interleukin-6 (Il-6) <br/>and tumor necrosis factor-? (Tnf-?) in blood of the immunocompromised mice. Fifty adult male Swiss albino mice were used in the present study; <br/>these were randomly divided into five groups (10 mice/each group) as follows: group I served as a healthy, uninfected control group; groups II-IV <br/>were immunosuppressed by dexamethasone, and uninfected, or infected (orally) with 3.0?103 (low dose) or 1.0?104 (high dose) Cryptosporidium parvum oocysts, <br/>respectively; group V did not receive dexamethasone and infected with 1.0x104 Cryptosporidium parvum oocysts. DNA damage of leucocytes was assessed <br/>by flow cytometry and comet assay; the gene expression of the cytokines was performed using real-time polymerase chain reaction. The results revealed <br/>that infection with the low or the high dose of Cryptosporidium parvum induced DNA damage in the leucocytes of the immunosuppressed mice, as revealed <br/>by the elevations in tail length and tail moment of the comet assay compared with the healthy and immunosuppressed, uninfected, mice. In addition, <br/>significant decreases and increases in the expression of Il-6 and Tnf-? genes, respectively, were recorded in leucocytes of the immunosuppressed, <br/>Cryptosporidium parvum-infected mice in comparison with the healthy and immuno-suppressed, uninfected, mice. In conclusion, Cryptosporidium infection <br/>induced DNA damage and augmented the changes induced in the Il-6 and Tnf-? gene expressions in the leucocytes of the immunosuppressed mice.<br/>Cryptosporidium parvum<br/>DNA damage<br/>IL-6<br/>Swiss albino mice<br/>TNF-?<br/><br/> 
<br/><br/><br/>Summary in Arabic 
<br/><br/><label>Subjects--Topical Terms: </label><br/>Cryptosporidium parvum	<br/>DNA Damage--physiology