Dhar, Sushant

Molecular characterization of new isolates of the entomopathogenic fungus beauveria bassiana and their efficacy against the tobacco caterpillar, spodoptera litura (fabricius) (lepidoptera: noctuidae) [electronic resource]. - p.60-68.

Includes reference.

Thirteen fungal isolates, having characteristics similar to Beauveria bassiana, were isolated from field soils of Punjab,
India, on Beauveria-specific selective medium (BS medium). Molecular screening of the B. bassiana isolates, using PCR amplification with B. bassiana-specific primer and nucleotide sequence analysis of ITS region, confirmed only three isolates as B. bassiana (now named as BbR1, BbR2, and BbR3) along with MTCC 2028 reference isolate. Comparative RAPD-PCR amplification with ten different RAPD primers showed that all the three isolates were closely related


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