TY  - SER
AU  - Ibrahim,A.H.
AU  - Abdeen,E.M.
TI  - Improving the efficiency of the traditional method of camel chymosin extraction using ultrasound and a freeze-drying process
KW  - Rennin
N1  - Includes bibliographic reference
N2  - Many problems are associated with the traditional extracting methods of camel chymosin; they are costly, time-consuming, 
and have low concentrations of chymosin. This study aimed to increase the efficiency of the traditional methods of camel 
chymosin production. Two experiments were carried out; the first experiment was implemented using dried camel abomasum 
as a powder and strips (4-7mm), and exposed to ultrasound exposure time at (20, 40, and 80 min). The chymosin extracted 
using the dried powder with ultrasound exposure time at 80 min treatments caused a higher significant increase in milk 
clotting activity and lower proteolytic activity compared to the strips and control treatments. The second experiment 
was implemented using the freeze-dried process for both chymosin extracted from the strips and powder treatments, 
which was selected as a higher in total clotting activity and chymosin yield. Upon comparison to the traditional samples, 
the freeze-dried treatments caused a higher significant increase in specific milk-clotting time and MCA/PA ratio 
by approximately 4.2 fold and 24.0 fold, respectively. Moreover, the freeze-dried process caused a significant increase 
in chymosin yield, curd viscosity, firmness, and reduced the syneresis of milk curd. Furthermore, the results confirmed 
that the viscosimetric measurement can be used as a new indicator to predict the cutting time of camel milk curd. 
The use of ultrasound with the freeze-dried process can be recommended as an appropriate treatment for the extraction 
of camel chymosin.

Keywords: Camel Chymosin, Ultrasound, Freeze-drying, Clotting activity, Proteolytic activity

UR  - http://nile.enal.sci.eg/EALE/2023/MUJFDS/1322/12/165.pdf
ER  -