Evaluation of breeding programs susceptibility for two important forage crops using DNA barcoding [electronic resource]
Language: English Summary language: Arabic Description: P. 28-37Other title:- تقييم قابلية نوعين من محاصيل العلف لبرامج التربية باستخدام تقنية DNA Barcoding [Added title page title]
- Alexandria science exchange journal, 2017 v. 38 (1) [electronic resource]:
| Item type | Current library | Call number | Status | Date due | Barcode | |
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Articles
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Main | ART ASEJ V38 No1 4 (Browse shelf(Opens below)) | Available |
Includes references.
This investigation was carried out to identify and evaluate Medicago sativa and Trifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes). Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes. Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively. Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1). Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively. Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.
Summary in Arabic.
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