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Evalvation of conventional pcr assay in comparison to real-time pcr assay for. Detection of bovine herpesvirus-l in bovine semen and seminal plasma [electronic resource].

By:

Darwish,Samah.F

Contributor(s):

Amin,A.S

Language: English Summary language: Arabic Description: p.285-299Other title:

تقيم اختبار تفاعل البلمرة المتسلسل التقليدى مقارنة باختبار تفاعل البلمرة المتسلسل الكمى للكشف عن فيروس الهربس البقرى1- فى كل من السائل المنوى وبلازما السائل المنوى البقرى [Added title page title]

Uniform titles:

Assiut veterinary medical journal, 2011 v. 57 (128) [electronic resource].

Subject(s):

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Full Text Article.

In: Assiut Veterinary Medical Journal 2011.v.57(128)Summary: The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays.

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The aim of this study was to evaluate the sensitivity of conventional Polymerase Chain Reaction (PCR) assay in comparison to real time PCR assays for detection of BoHV -1 in bovine semen and seminal plasma. Tenfold serial dilutions of BoHV-l strain were prepared in one ml aliquots of both minimal essential medium (MEM) and negative bovine semen. The dilutions started from 107 to 10-4 to detect detection limits of both PCR and real time PCR assays.

Summary in Arabic.

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