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Detection of DNA damage of Clarias gariepinus exposed to 2, 4-D using chromosomal aberrations and RAPD assays [electronic resource].

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Abumourad, I. M

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Language: English Summary language: Arabic Description: p.685-700Other title:

اكتشاف تلف الحمض النووي دنا الناتج عن استخدام مبيد التوفوردى2, 4-D باستخدام تقنية الشذوذات الكرموسومية والتضخيم العشوائى لقطع الدنا(الرابيد) فى اسماك قراميط المياه العذبه [Added title page title]

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Bulletin of Faculty of Agriculture. Cairo University, 2006 v. 57 (4) [electronic resource].

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In: The bulletin. Faculty of Agriculture. Cairo University 2006.v.57(4)Summary: The widely used herbicide 2, 4- dichorophenoxy acetic acid (2, 4- D) is evaluated for acute toxicity and stress factors on fresh water fish. In this study, induced chromosomal aberrations in vivo were studied using three concentrations of 2, 4 -D (10, 20 and 30 mg/ml) for 7 days, the percentage of chromosomal aberrations was found to be statistically highly significant after treatment with the different doses. RAPD (PCR- based diagnostic assay) was used also as a bioindicator of the pollutant's toxicity to assess the genetic damage applied on primary liver and spleen cell culture of Clarias gariepinus using different concentrations of the pollutant (0.05.0.1 and 0.5 ug/ml). RAPD- PCR was applied using six primers. The PCR results demonstrated genetic different damage in the RAPD fingerprinting as a result of toxicity; these differences may be due to selection pressure of pollutant on fish.

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The widely used herbicide 2, 4- dichorophenoxy acetic acid (2, 4- D) is evaluated for acute toxicity and stress factors on fresh water fish. In this study, induced chromosomal aberrations in vivo were studied using three concentrations of 2, 4 -D (10, 20 and 30 mg/ml) for 7 days, the percentage of chromosomal aberrations was found to be statistically highly significant after treatment with the different doses. RAPD (PCR- based diagnostic assay) was used also as a bioindicator of the pollutant's toxicity to assess the genetic damage applied on primary liver and spleen cell culture of Clarias gariepinus using different concentrations of the pollutant (0.05.0.1 and 0.5 ug/ml). RAPD- PCR was applied using six primers. The PCR results demonstrated genetic different damage in the RAPD fingerprinting as a result of toxicity; these differences may be due to selection pressure of pollutant on fish.

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