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Molecular characterization of an Egyptian isolate of tomato mosaic tobamovirus [electronic resource].

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EI-Mfifi, Sobair L

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Language: English Summary language: Arabic Description: p.467-483Other title:

الخصائص الجزيئية لعزلة مصرية من فيروس موزيك الطماطم [Added title page title]

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Annals of agricultural science, 2004 v. 49 (2) [electronic resource].

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In: Annals of Agricultural Science 2004.v.49(2)Summary: Tomato plant is recorded to be one of the most economical vegetable crops in Egypt This crop was found to be widely and severely infected with tomato mosaic: tobamoviTus (ToMV). An Egyptian isolate of ToMV was subjected to molecular characterization. The virus was purified from tobacco leaves using differential centrifugation. The electron microscopy of purified vims ptepaIlltion showed the presence of rod-shaped vims-like particles with a model size close to 300 X 17 om. The SOS-polyacrylamide gel electrophoresis (SOS-PAGE) showed a single coat protein band with molecular weight of ahout 17 KDa ToMV coat protein gene (cp) was is0lated and amplified using immunocaptwe reverse transeriptase polymerase chain reaction (IC-RT-PCR) and its size length on agarose gel was found to be 479 bp. The nucleotide sequence of the viml genome was studied, as a genome composed of 6383 nucleotides was sequenced and its organization was addressed.

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Tomato plant is recorded to be one of the most economical vegetable crops in Egypt This crop was found to be widely and severely infected with tomato mosaic: tobamoviTus (ToMV). An Egyptian isolate of ToMV was subjected to molecular characterization. The virus was purified from tobacco leaves using differential centrifugation. The electron microscopy of purified vims ptepaIlltion showed the presence of rod-shaped vims-like particles with a model size close to 300 X 17 om. The SOS-polyacrylamide gel electrophoresis (SOS-PAGE) showed a single coat protein band with molecular weight of ahout 17 KDa ToMV coat protein gene (cp) was is0lated and amplified using immunocaptwe reverse transeriptase polymerase chain reaction (IC-RT-PCR) and its size length on agarose gel was found to be 479 bp. The nucleotide sequence of the viml genome was studied, as a genome composed of 6383 nucleotides was sequenced and its organization was addressed.

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