000 02538cam a2200289Ia 45 0
001 u200652
003 SIRSI
008 101026s2006 ua ss b 1 eng d
040 _aEAL
041 _aeng
_bara
050 _aART ESAMP No1 9
100 1 _aAbdel Gawad A. M.
240 1 0 _aProceedings of the 1st scientific congress
_h[electronic resource]:
_bthe role of veterinary medicine in animal, and poultry, and fish wealth development : Giza, Egypt 30 -31 October, 2006.
245 1 0 _aVaccination of dogs against Rhipicephalus sanguineus ticks using salivary gland antigen
_h[electronic resource].
246 1 5 _aتحصين الكلاب ضد قراد الريباسيفليس سنجوينس باستخدام مستضد الغدد اللحابية.
300 _ap.167-179.
504 _aIncludes references.
520 _aThe present study aimed to induce experimental resistance in dogs against Rhipicephalus sanguineus ticks using salivary gland antigen. Four dogs were vaccinated by salivary gland antigen obtained from partially fed adult ticks then challenged with 10 pairs of unfed adult ticks on the 35th day. The fifth dog remained as a control inoculated with adjuvant only. The results of vaccination showed a high protection in terms of de¬creased the percentage of tick attachment and significant reduction in the weight of engorged female ticks. SDS-PAGE analysis of salivary gland antigen revealed 14 bands ranging from 119.7 to 38 KDa. Immunoblotting with hyper-immune sera against salivary gland antigen showed 4 polypep¬tides. In contrast only three bands were detected with naturally infested dog sera, Three common polypeptides were recognized in both sera with molecular weights 97, 75 and 38 KDa, but two of them (97 and 38 Kda) were strongly recognized with hyper immune sera and faintly in naturally infested sera. One band of 88 KDa was recognized by hyper-immune sera only. Therefore, tick salivary gland antigen seem to have promising poten¬tials in inducing resistance in dogs.
546 _aSummary in Arabic.
650 0 _aDogs
_xDiseases.
650 0 _aDogs
_xVaccination.
650 0 _aRhipicephalus sanguineus.
700 1 _aEl-Kelesh, Eman, A. M.
773 0 _tProceedings of the 1st Scientific Congress:the role of veterinary medicine in animal, and poultry, and fish wealth development : Giza, Egypt 30 -31 October, 2006
_dCairo:Egyptian Society for Animal Management,2006.
_7nnam
_wu200583
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2006/EJAPM/106/1/167.pdf
_zFull Text Article.
596 _a1
942 _cBK
_2lcc
999 _c35934
_d35934