000			02760cab a2200301Ia 45 0
001			u185053
003			SIRSI
008			100919s2007 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART MUJAS V32 No6 25
100	1		_aEl-Shihy, O.M.
240	1	0	_aMansoura University journal of agricultural sciences, 2007 v. 32 (6) _h[electronic resource].
245	1	0	_aIn vitro short-term germplasm preservation of Myrtus communis L. _h[electronic resource].
246	1	5	_aالحفظ قصير الاجل للاصل الوراثي لشجيرة الاس معمليا.
300			_ap.4451-4473
504			_aIncludes reference.
520			_aShoot tips were obtained from Myrtus communis tissue cultured shootJets and in vitro preserved either by cryopreservation protocols (through vitrification, encapsulation-dehydration) or by cold preservation (4°C). as well as control storage at culture room conditions, however, nodal stem was involved in dehydration protocol in order to be cryopreserved. Preservation was adopted for 1, 2 and 3 months durations; yet, control treatment was prolonged for one year. Several cryoprotection trials were adopted. involving DMSO and/or PEG and glycerol. prior to vitrification protocol. Cryopreserved explant recovery was tested. as well as shoot/ets regeneration following preservation processes underwent several investigations. Shoot tips precultured on 0.18 M sucrose recorded higher recovered survival. shootlets number, length and leaves number than on a higher concentration and than on mannitol, either for 2 or 4 days. however. vitrification treatment induced no shootlets proliferation. Regenerated shooUets growth characters (survival %, shootlets number and dry weights) induced after preservation under growth chamber conditions (control) for Cine year, recorded the highest observed results. Similarly, chemical composition (chlorophyll a, total chlorophylls. carotenoids, total indoles. total free amino acids, total sugars and indolesJphenols ratio) as well as essential oil content and components were augmented due to preservation under control conditions; however, fresh weight, chlorophyll b, as well as total soluble phenols were low. RAPD analysis proved that preserved shootlets maintained their genetic stability through germplasm preservation methods.
546			_aSummary in Arabic.
650		0	_aOrnamental woody plants.
650		3	_aMyrtus communis
700	1		_aGhallab, A.M.
700	1		_aGad, Mervat M.A.
700	1		_aAbdel Salam, Ranya R.
773	0		_tMansoura University Journal of Agricultural Sciences. _g2007.v.32(6) _x1110-0346 _7nnas _wu182197
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2007/MUJAS/3207/6/4451.pdf _zFull Text Article.
596			_a1
942			_cAR _2lcc
999			_c40461 _d40461