| 000 | 02573cab a2200289Ia 45 0 | ||
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| 001 | u186259 | ||
| 003 | SIRSI | ||
| 008 | 101020s2009 ua ss b eng d | ||
| 040 | _aEAL | ||
| 041 |
_aeng _bara |
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| 090 | _aART EJH V36 No1 8 | ||
| 100 | 1 | _aEI-Sharny, M. A. | |
| 240 | 1 | 0 |
_aEgyptian journal of horticulture, 2009 v. 36 (1) _h[electronic resource]. |
| 245 | 1 | 0 |
_aMicropropagation and genetic stability of Pyracantha fortuneana, roem. shrub _h[electronic resource]. |
| 246 | 1 | 5 | _aالاكثار الدقيق والثبات الوراثى لشجيرة البيراكانثا Pyracantha Fortuneana |
| 300 | _ap.149-161. | ||
| 504 | _aIncludes references. | ||
| 520 | _aThis study was carried out in Plant Tissue Culture Laboratory at EI-Zohria Botanical Garden, Horticulture Research Institute, Agriculture Research Center during 2007-2008 seasons. The object of this study wa:; to establish a protocol for micropropagation and RAPD analysis for comparing between mother plant and in vitro plant of Pyracantha fortuneana Roem. The data revealed that for sterilization explant, using 40% c1orox for 25 min gave the best result (100% survival) and free contamination explant. Bud break and shoot development were best on full MS establishment medium with (2.0 mgll IBA) which gave the longest shootlet. While the greatest number of leaves was recorded on full MS-medium with (4.0 mglIBA). For shooting behaviour. the medium supplemented with (3.0 mgll BA) for four subcultures gave the greatest number of shootlet. But adding (4.0 or 5.0 mgtl kin) for four subcultures produced the tallest shoodet length. For the number of leaves. culture the explant on MS-medium have (4.0 mgt/ BA) for four subcultures gave the highest number of leaves. Regarding to the rooting behaviour, culturing the shootlet on MS-medium supplemented with (4.0 mg/lBA) produced the highest percentage of rooting, tallest root length and highest number of root. RAPD-based DNA fingerprints gave no evidence of somaclonal variation during the in vitro propagation protocol used. The RAPD profiles of tissue culture-derived plantlets revealed high similarity to mother tree. For acclimatization. the survival percentage was 70%. | ||
| 546 | _aSummary in Arabic. | ||
| 650 | 0 | _aPyracantha. | |
| 650 | 0 |
_aPyracantha _xMicropropagation. |
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| 700 | 1 | _aSayed, Sawsan S. | |
| 700 | 1 | _aGomaa, S. A. A. | |
| 773 | 0 |
_tEgyptian journal of horticulture. _g2009.v.36(1) _x1110-0206 _7nnas _wu183584 |
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| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2009/EJH/3609/1/149.pdf _zFull Text Article. |
| 596 | _a1 | ||
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_c41509 _d41509 |
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