000 01959cab a2200337Ia 45 0
001 u186694
003 SIRSI
008 101102s2007 ua ss b eng d
040 _aEAL
090 _aART JGEB V5 No1-2 5
100 1 _aTalkhan, Fatma N.
240 1 0 _aJournal of genetic engineering and biotechnology, 2007.v. 5 (1-2)
_h[electronic resource].
245 1 0 _aEffect of UV treatments on the activity of delta-endotoxin protein crystals in Bacillus thuringiensis
_h[electronic resource].
300 _ap.33-42.
504 _aIncludes references.
520 _aThe ability of some isolates of B.thuringiensis to produce dark brown pigment were measured as an indicator to UV resistant, Ms isolate was used as wild type to improve it to be more resistant to UV, upon. It exposed to UV irradiation with different periods, ranged between 1 to 10 hrs. The induced mutants were examined morphologically by phase contrast microscope. 144 mutants were obtained, ten ofthem were selected and tested for their toxicity against Spodoptera littorallis. The results showed that mutants 62, 64 and 85 were the most toxic ones. These three mutants and wild type were examined by transmission electron microscope. Crystal proteins, with bipyramidial shape and active against Lepidoptera insects, were detected in all the selected mutants.
650 0 _aBacillus thuringiensis.
650 0 _aBacillus thuringiensis
_xToxicology
650 0 _aEndotoxins
_xPhysiological effect.
650 0 _aUltraviolet radiation.
650 3 _adelta-endotoxins.
650 3 _aentomopathogenic bacteria.
700 1 _aAbo-assy, H. H.
700 1 _aSoliman, Kh. A.
700 1 _aAbdel-Razek, A. S.
700 1 _aAzzam, Marwa M.
700 1 _aAbdelsalam, A. Z. E.
773 0 _tJournal of genetic engineering and biotechnology.
_g2007.v.5(1-2)
_x1687-157X
_7nnas
_wu186667
856 4 0 _uhttp://nile.enal.sci.eg/EALE/2007/JGEB/507/1/33.pdf
_zFull Text Article
596 _a1
942 _cAR
_2lcc
999 _c41810
_d41810