000			02289cab a2200301Ia 45 0
001			u187604
003			SIRSI
008			101208s2006 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART BFACU V57 No4 7
100	1		_aAbumourad, I. M,
240	1	0	_aBulletin of Faculty of Agriculture. Cairo University, 2006 v. 57 (4) _h[electronic resource].
245	1	0	_aDetection of DNA damage of Clarias gariepinus exposed to 2, 4-D using chromosomal aberrations and RAPD assays _h[electronic resource].
246	1	5	_aاكتشاف تلف الحمض النووي دنا الناتج عن استخدام مبيد التوفوردى2, 4-D باستخدام تقنية الشذوذات الكرموسومية والتضخيم العشوائى لقطع الدنا(الرابيد) فى اسماك قراميط المياه العذبه.
300			_ap.685-700.
504			_aIncludes references.
520			_aThe widely used herbicide 2, 4- dichorophenoxy acetic acid (2, 4- D) is evaluated for acute toxicity and stress factors on fresh water fish. In this study, induced chromosomal aberrations in vivo were studied using three concentrations of 2, 4 -D (10, 20 and 30 mg/ml) for 7 days, the percentage of chromosomal aberrations was found to be statistically highly significant after treatment with the different doses. RAPD (PCR- based diagnostic assay) was used also as a bioindicator of the pollutant's toxicity to assess the genetic damage applied on primary liver and spleen cell culture of Clarias gariepinus using different concentrations of the pollutant (0.05.0.1 and 0.5 ug/ml). RAPD- PCR was applied using six primers. The PCR results demonstrated genetic different damage in the RAPD fingerprinting as a result of toxicity; these differences may be due to selection pressure of pollutant on fish.
546			_aSummary in Arabic.
650		0	_aClarias gariepinus _xEffect of pesticides on.
650		0	_aDNA damage _xResearch
650		0	_aChromosome abnormalities.
700	1		_aEI- Sherbeny, K. M.
700	1		_aFarghaly, A. A.
773	0		_tThe bulletin. Faculty of Agriculture. Cairo University _g2006.v.57(4) _x0526-8613 _7nnas _wu158095
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2006/BFACU/5706/4/685.pdf _zFull Text Article
596			_a1
942			_cAR _2lcc
999			_c42554 _d42554