000			01902cab a2200337Ia 45 0
001			u190159
003			SIRSI
008			110309s2007 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART ZVJ V35 No1 12
100	1		_aSoliman, Y. A.
240	1	0	_aZagazig veterinary journal, 2007 v. 35 (1) _h[electronic resource].
245	1	0	_aMolecular cloning and expression of ESAT-6 antigen of Mycobacterium bovis for differential diagnosis of bovine tuberculosis _h[electronic resource].
246	1	5	_aكلونة وتعبير الأنتيجين ESAT-6 من ميكروب السل البقرى للتشخيص التفاضلى للسل البقرى.
300			_aP. 122-134.
504			_aIncludes references.
520			_aIn a search for developing new skin test reagents, the possibility of using ESAT-6 protein antigen as a candidate antigen for the diagnosis of bovine tuberculosis was investigated. Genomic DNA of M. bovis was extracted, purified and the esat-6 gene was amplified by PCR. The gene was then ligated to an expression vector, PQE. After transformation of an E. coli TOPO host strain with the PQE plasmid, the expression was induced using 10 mM of IPTG. A band (18 KDa) was seen in the SDS-PAGE analysis. The His-tagged r ESAT-6 antigen was then purified by metal affinity chromatography using Ni-NTA agarose.
546			_aSummary in Arabic.
650		0	_aTuberculosis in cattle.
650		0	_aMycobacterium bovis.
650		0	_aMolecular cloning.
650		0	_aTuberculosis _xMicrobiology.
650		0	_aMycobacterium bovis.
650		0	_aEnzyme-linked immunosorbent assay.
650		0	_aTuberculosis _xDiagnosis.
650		0	_aAntigen-antibody reactions.
773	0		_tZagazig Veterinary Journal. _g2007.v.35(1) _x1110-1458 _7nnas _wu189860
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2007/ZVJ/3507/1/122.pdf _zFull Text Article.
596			_a1
942			_cAR _2lcc
999			_c44760 _d44760