000			02422cab a2200313Ia 45 0
001			u190730
003			SIRSI
008			110328s2009 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART ZVJ V37 No4 7
100	1		_aAli, Ahmed Abdel Samie H.
240	1	0	_aZagazig veterinary journal, 2009 v. 37 (4) _h[electronic resource].
245	1	0	_aVirological and molecular studies on rotavirus _h[electronic resource].
246	1	5	_aدراسات فيرولوجية وجزيئية على فيروس الروتا.
300			_ap.70-79.
504			_aIncludes references.
520			_aBovine Rotavirus (BRV) is a major cause of acute gastroenteritis in neonatal calf diarrhea throughout the world causing economic losses to beef and dairy industries. Neomycin B, an aminoglycoside antibiotic has been shown to inhibit human immunodeficiency virus and Herpes simplex virus replication. The current study was carried out to assess the effect of neomycin B on rotavirus replication in Rhesus monkey kidney fetal cells (MA-104) by immunofluorescence (IF), plaque assay and now cytometry. Neomycin interfered with the development of BRV cytopathic effect (CPE). Neomycin concentrations at 5, 7.5 and 10 mM were able to reduce the intracytoplasmic fluorescence induced by BRV byI6.67%. 41,67% and 83.33% respectively. There was marked decrease in the yield of viral RNA. Neomycin concentrations of 5. 7.5 and 10mM were observed to be able to reduce the percentage of cell infectivity with BRV by 16.21 %. 56.72% and 73.94% respectively. The kinetics of BRV growth was carried out by plaque assay. At the concentrations 7.5 and 10mM neomycin. there was marked decrease in virus titer in supernatant at time interval 48 and 72h post infection, It was concluded that Neomycin B is an inhibitor of BRV replication in-vitro particularly when the drug IS added during the complete infection cycle; pre-incubation of virus and pretreatment of MA-104 with neomycin does not affect replication of BRV.
546			_aSummary in Arabic.
650		0	_aRotaviruses.
650		0	_aCattle _xDiseases
650		0	_aNeomycin.
700	1		_aSalama, Ali A.
700	1		_aZeidan, Sayed M.
700	1		_aMansour, Shimaa M.G.
773	0		_tZagazig Veterinary Journal. _g2009.v.37(4) _x1110-1458 _7nnas _wu189860
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2009/ZVJ/3709/4/70.pdf _zFull Text Article
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