000			02143cab a2200325Ia 45 0
999			_c45511 _d45511
001			u191006
003			SIRSI
008			110405s2010 ua ss b eng d
040			_aEAL
041			_aeng _bara
090			_aART AVMJ V56 NO125 11
100	1		_aBassiony, M. M. _92792
240	1	0	_aAssiut veterinary medical journal, 2010 v. 56 (125) _h[electronic resource].
245	1	0	_aDiagnosis of brucellosis in lactating ewes using milk-elisa _h[electronic resource].
246	1	5	_aتشخيص مرض البروسيلا فى الاغنام الحلابة بأستخدام اختبار الاليزا فى اللبن.
300			_ap.143-153.
504			_aIncludes references.
520			_aMilk and blood samples collected from 731 lactating ewes were examined for brucellosis using milk-ELISA, serum-EUSA, RBPT, SAT and MRT. Of these ewes, 150 were from brucellosis free sheep flock, which were found negative in all tests used. While, of the remaining 581 ewes, III were positive in serum-ELISA, 107 in milk-ELISA, 96 in RBPT, 85 in SAT, 48 in MRT and B.melitensis biovar 3 was isolated from the milk of 39 infected ewes. Of the 111 serum-ELISA positive ewes, 101 were positive and 10 were negative in milk-ELISA, whereas of the 107 milk-ELISA positive ewes, 6 ewes were negative in scrum-ELISA. All together, 101 ewes were positive and 464 were negative in both the assays, while 16 ewes yield variable results. The sensitivity and positive predictive value in the infected flocks were 94.39% and 90.99% respectively. The milk-ELISA for diagnosing brucellosis seems to be an attractive alternative of serum-ELISA particularly in the lactating ewes.
546			_aSummary in Arabic.
650		0	_aEwes _xDiseases. _9340
650		0	_aSheep milk. _93153
650		0	_aBrucellosis in sheep _xDiagnosis. _9379
650		0	_aEnzyme-linked immunosorbent assay. _9368
700	1		_aAbou gazia, K. A. _93154
700	1		_aAbd al-azeem, M. W. _93155
700	1		_aIbrahim, I. G. A. _93156
773	0		_tAssiut Veterinary Medical Journal. _g2010.v.56(125) _x1012-5973 _7nnas _wu181356
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2010/AVMJ/5610/125/143.pdf _zFull Text Article.
942			_cAR _2lcc