| 000 | 02583cab a2200337Ia 45 0 | ||
|---|---|---|---|
| 001 | u191639 | ||
| 003 | SIRSI | ||
| 008 | 110427s2007 ua ss b eng d | ||
| 040 | _aEAL | ||
| 041 |
_aeng _bara |
||
| 090 | _aART AJB V10 No2 13 | ||
| 100 | 1 | _aEL-Sabagh, I. M. | |
| 240 | 1 | 0 |
_aArab journal of biotechnology, 2007 v. 10 (2) _h[electronic resource]: _b an international journal devoted to the advancement of biotechnology. |
| 245 | 1 | 0 |
_aConstruction of a recombinant baculovirus expressing the major capsid protein (VP6) of bovine rotavirus _h[electronic resource]. |
| 246 | 1 | 5 | _aبناء فيروس مدمج من جنس الباكيلو للتعبير عن بروتين الغلاف الداخلى لفيروس الروتا البقرى. |
| 300 | _ap.369-384. | ||
| 504 | _aIncludes references. | ||
| 520 | _aThe present study reports the expression of VP6, the major inner capsid protein of bovine rotavirus Nebraska calf diarrhea virus (NCDV) strain in a baculovirus expression system. The fulllength DNA copies of RNA segment 6 (coding for VP6 protein) of NCDV were inserted into a baculovirus expression vector. A recombinant baculovirus carrying the VP6 gene was constructed through homologous recombination between the baculovirus recombinant plasmid carrying the VP6 gene and Autographa californica nuclear polyhedrosis virus (AcNPV) under the control of the polyhedrin promotor. Infection of Spodoptera frugiperda (Sf9) cells with the recombinant baculovirus expressing VP6 protein revealed a high-level of expression when tested by immunoflurescence and solid phase ELISA tests using BRV-specific polyclonal antibodies. The VP6 expressed protein was detected in Coomassie blue stained SDS-PAGE and produced a detectable band in Western blot assay. The high degree of reactivity with BRV-specific polyclonal antibodies confirmed that the antigenic determinants of the expressed protein were unaltered. The use of the in vitro expressed VP6 protein in the field diagnosis and vaccine development to control rotavirus infection is of considerable interest. | ||
| 546 | _aSummary in Arabic. | ||
| 650 | 0 | _aRotaviruses. | |
| 650 | 0 |
_aBaculoviruses _xBiotechnology. |
|
| 650 | 0 |
_aBaculoviruses _xGenetics _xLaboratory manuals. |
|
| 700 | 1 | _aHussein, H. A. | |
| 700 | 1 | _aAmer, H. M. | |
| 700 | 1 | _aEL-Sanousi, A. A. | |
| 700 | 1 | _aReda, I. M. | |
| 700 | 1 | _aShalaby, M. A. | |
| 773 | 0 |
_tArab journal of biotechnology. _g2007.v.10(2) _x1110-6875 _7nnas _wu189256 |
|
| 856 | 4 | 0 |
_uhttp://nile.enal.sci.eg/EALE/2007/AJB/1007/2/369.pdf _zFull Text Article |
| 596 | _a1 | ||
| 942 |
_cAR _2lcc |
||
| 999 |
_c46076 _d46076 |
||