000			01896cab a2200313Ia 45 0
999			_c46168 _d46168
001			u191798
003			SIRSI
008			110503s2009 ua ss b 0 eng d
040			_aEAL
041			_aeng _bara
090			_aART AVMJ V55 NO122 22
100	1		_aAbd-Allah, S. M. _92720
240	1	0	_aAssiut veterinary medical journal, 2009 v. 55 (122) _h[electronic resource].
245	1	0	_aSuccessful production of buffalo embryos from vitrified immature and mature oocytes in vitro _h[electronic resource].
246	1	5	_aإنتاج ناجح لاجنة الجاموس من بويضات غير ناضجة وناضجة مزججة بالتجميد السريع معمليا.
300			_ap.332-343.
504			_aIncludes references.
520			_aThe current study was conducted to produce buffalo embryos in vitro from vitrified immature and mature oocytes. Cumulus oocytes romplexes (COCs) wm obtained from ovaries whil>h collel>ted from l! local abattoir Good quality COCs (immature oocytes) were vitrified immediately after collection. After 22-24 hours ofCOCs culture oocytes were vitrified similarly. All oocytes (immature and marure) were exposed to vitrification solution of different concentnltions (glycerol: 3.3,6.6 and 1000Ao) at 5, 5 and 10 minutes intervals respectively. The oocytes were finally placed in 10% glycerol and Immediately loaded in 0.25 ml plastic stnlws, each containing 100 )ul of 10% sucrose in Modified phosphate buffer saline (M-PBS).
546			_aSummary in Arabic.
650			_aBuffaloes _x Embryos _996
650		0	_aBuffaloes _xReproduction. _996
650		2	_aOocytes. _91665
650		2	_aCryopreservation _xmethods. _91436
700	1		_aGomaa,A. _92721
700	1		_aAbd El-Gawad,E.M.M. _92722
773	0		_tAssiut Veterinary Medical Journal. _g2009.v.55(122) _x1012-5973 _7nnas _wu181356
856	4	0	_uhttp://nile.enal.sci.eg/EALE/2009/AVMJ/5509/122/332.pdf _zFull Text Article.
942			_cAR _2lcc